American Society of Hematology

Fig. 1.

$Fig. 1. A flow cytometric analysis of lineage-specific antigens and CD34 expression on human cord blood cells and their purification. Staining profile of lineage markers and CD34 versus CD45 expression on human cord blood MNCs before (A) and after filtration (B). (C) Sorted Lin−CD34− cells show a more than 99% pure fraction. (D) Isotype antibody was used as a negative control. (E) Semiquantitative RT-PCR analysis from CD34−Lin− cells. Top lane, human CD34 cDNA amplification; bottom lane, GAPDH cDNA amplification used to normalize RT-RNAs; PC, RT-PCR products from KG-1 cells; NC, water; CD34−, sorted CD34− cells; CD34+, sorted CD34+ cells; 10−1, 10−2, 10−3, 10−4, and 10−5, ratio of positive and negative cell; 10−5, mean positive and negative cell ratio is 1:105.$

A flow cytometric analysis of lineage-specific antigens and CD34 expression on human cord blood cells and their purification. Staining profile of lineage markers and CD34 versus CD45 expression on human cord blood MNCs before (A) and after filtration (B). (C) Sorted Lin⁻CD34⁻ cells show a more than 99% pure fraction. (D) Isotype antibody was used as a negative control. (E) Semiquantitative RT-PCR analysis from CD34⁻Lin⁻ cells. Top lane, human CD34 cDNA amplification; bottom lane, GAPDH cDNA amplification used to normalize RT-RNAs; PC, RT-PCR products from KG-1 cells; NC, water; CD34⁻, sorted CD34⁻ cells; CD34⁺, sorted CD34⁺ cells; 10⁻¹, 10⁻², 10⁻³, 10⁻⁴, and 10⁻⁵, ratio of positive and negative cell; 10⁻⁵, mean positive and negative cell ratio is 1:10⁵.

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