Extent of CD34⁺ cell engraftment in NOD/SCID mice after attachment and detachment.

Mouse bone marrow mononuclear cells were immunostained with antihuman CD45^FITC and CD34^PE mAbs and analyzed by FACS by using Cell Quest Software (Becton Dickinson). The percentage CD45⁺ cells was determined on ungated samples. (A) Plot of the percentage CD45⁺ cells in mice injected with cells plated on FN in the presence (diamonds) or absence (control; triangles) of TS2/16.2.1 activating mAb. (B) Scatter plot of the percentage of CD45⁺ cells in mice injected with RN-attached cells (circles) or control cells (ie, cells maintained in suspension or plated on FN in the absence of activating mAb; inverted triangles). Horizontal lines represent the mean percentage CD45⁺ cells in each group.