Microinjected CD34⁺ cells are viable and retain their ability to proliferate after single cell transfer.

Cells were attached to RN and then microinjected with OG-dextran. Two hours after injection, cells were detached with peptide, and the fluorescent cells were transferred as single cells into individual wells of 96-well plates (C). Cells in suspension (A) and cells attached to RN and detached with peptide (B) were also transferred as single cells. Survival and proliferation of the cells was monitored at 0, 2, and 6 days after transfer. Values shown represent the percentage of total wells transferred that displayed cells surviving (closed bar) and proliferating (open bar) for 1 experiment in which 30 suspension, 30 RN attached/peptide detached, and 45 OG-dextran microinjected CD34⁺ cells were transferred.