Nuclear Rel proteins are differentially expressed during generation and maturation of human dendritic cells and development of macrophages in vitro.

Immunoblots were performed with nuclear proteins from monocytes (day 0, lanes 1 and 4), immature dendritic cells (day 7, lane 2), mature dendritic cells (day 10, lane 3), differentiated macrophages (day 7, lane 5), and macrophages treated with monocyte-conditioned medium for another 3 days (day 10, lane 6). Proteins were separated on an 8% sodium dodecyl sulfate–polyacrylamide gel and electrophoretically transferred to a nitrocellulose membrane.