Comparison of secondary lymphoid-tissue chemokines (SLC)- and EBI1-ligand chemokine (ELC)-induced adhesion of adult T-cell leukemia (ATL) cells from patients with and without lymphoid organ involvement to ICAM-1 under flow conditions.
Cells (106/ml) were perfused onto the coated dish with substrates consisting of ICAM-1 (10 μg/ml) alone or also with SLC (10 μg/ml) or ELC (10 μg/ml) at 1 dyne/cm2 for the first 4 minutes. The number of arrested cells is shown by the total number accumulated as arrested cells for the first 4 minutes in a single field of view (4X objective). The cells are as follows: control CD4+CD45RO+ T cells (A, closed squares) and ATL cells from patients without (B, patients 1 through 12) and with lymphoid organ involvement (C, patients 13 through 28). ATL cells from patients with lymphoid organ involvement were blocked with 100 ng/ml pertussis toxin (PTX) for 1.5 hours at 37°C (C+PT) or with anti-CD18 mAb, 7E4 (20 μg/ml) for 20 minutes at room temperature (C+CD18 Ab) before binding to ICAM-1 with SLC or ELC. Experiments were performed in triplicate. The bars indicate the mean value of each group; the statistical analysis was performed using Student ttest.
