Comparison of secondary lymphoid-tissue chemokine (SLC)- and EBI1-ligand chemokine (ELC)-evoked chemotaxis of adult T-cell leukemia (ATL) cells from patients with and without lymphoid organ involvement.

Chemotactic assays were performed in the presence of SLC or ELC at a low concentration (30 ng/ml) as described in Materials and Methods. The cells are as follows: control CD4⁺CD45RO⁺ T cells (A, closed squares) and ATL cells from patients without (B, patients 1 through 12) and with lymphoid organ involvement (C, patients 13 through 28). ATL cells from patients with lymphoid organ involvement were blocked with 100 ng/ml pertussis toxin (PTX) for 1.5 hours at 37°C (C+PT) before chemotactic assays. The data are expressed as the mean percentage of migrated cells toward SLC or ELC from triplicate experiments. The bars indicate the mean value of each group; the statistical analysis was performed using Student t test.