![Fig. 1. Effect of ADA on fMLP- and MSU-crystals–induced PLD activity. / Neutrophils labeled with 1-O-[3H]alkyl-2-lysophosphatidylcholine were prewarmed at 37°C for 5 minutes. (A) The cell suspensions were pretreated with 10 μmol/L CB and incubated for an additional 5 minutes in the absence or presence of 0.1 U/mL ADA. Cells were stimulated with 0.1 μmol/L fMLP in the presence of 1% ethanol for 10 minutes. The amount of radioactivity incorporated into PEt was measured as described in “Materials and Methods.” The levels of [3H]PEt formed are expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 6 separate experiments. (B) Cell suspensions were stimulated with 3 mg/mL MSU crystals in the presence of 1% ethanol for 15 minutes, without CB. The data are the means ± SEM of 4 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.519/6/bloo00203001x.jpeg?Expires=1735506016&Signature=ovPQ-tDyIpxRcqFACnM1Bak2uLXKEQ~tblu-qLz2BzBEuBR2LNqd~6MAl4cHZPJ1aMH017XrZ5FhEP-UoEPd~ro6mw06u7NmS~W066HZFhohzbRdUyYSGZ~G0XQM0bYgwR3Q3XdsEfk0W-y0iO6MAWzxVs1ug4zydkCwodjFFjVXK25vdnrcl8-1feQ8pqXmlZ43G7xOvIyZNB4fAj3DIql4blItiSn~kYoHb9YYMHC14Gl9k5SRmwzN4fOw~eR-U0YVeV2UTCJEuBjwTzXPCJQ2oYjQeVI7cIlacxlTDWGq9p9pZqHbpkI6BhmIGFXvjyETZty9FgJqKGSPLbC-jQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of ADA on fMLP- and MSU-crystals–induced PLD activity.
Neutrophils labeled with 1-O-[3H]alkyl-2-lysophosphatidylcholine were prewarmed at 37°C for 5 minutes. (A) The cell suspensions were pretreated with 10 μmol/L CB and incubated for an additional 5 minutes in the absence or presence of 0.1 U/mL ADA. Cells were stimulated with 0.1 μmol/L fMLP in the presence of 1% ethanol for 10 minutes. The amount of radioactivity incorporated into PEt was measured as described in “Materials and Methods.” The levels of [3H]PEt formed are expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 6 separate experiments. (B) Cell suspensions were stimulated with 3 mg/mL MSU crystals in the presence of 1% ethanol for 15 minutes, without CB. The data are the means ± SEM of 4 separate experiments.
