Fig. 2.
Effect of ADA on fMLP-stimulated translocation of PKCα, RhoA, and ARF1 to membranes.
Unlabeled neutrophils were prewarmed 5 minutes at 37°C. (A) 10 μmol/L CB was added and the cell suspension incubated for an additional 5 minutes in the presence or absence of 0.1 U/mL ADA. Neutrophils were stimulated with 0.1 μmol/L fMLP for 2 minutes. (B) Cell suspensions were stimulated with 3 mg/mL MSU crystals for 15 minutes, without CB. Incubations were stopped and neutrophil membranes prepared as described in “Materials and Methods.” The samples were analyzed for PKCα, RhoA, and ARF1 by immunoblotting. The data are from 1 experiment representative of 3 similar experiments.
