Fig. 3.
Time course of Ado accumulation in neutrophil suspensions.
Neutrophils were resuspended in HBSS at the concentration of 2 × 107 cells/mL and incubated at 37°C for 13 minutes before the addition of 0.001, 0.01, and 0.1 U/mL ADA. At selected times, aliquots (1 mL) of the cell suspensions were denatured and processed for measurement of Ado content by liquid chromatography-mass spectrometry. The data are the means ± SEM of triplicate incubations from 1 experiment representative of 3 similar experiments.
