Fig. 4.
![Fig. 4. Effect of Ado receptor agonists on fMLP-induced PLD activity. / 1-O-[3H]alkyl-2-lysophosphatidylcholine-labeled neutrophils were preincubated at 37°C for 5 minutes. The cell suspensions were then pretreated with 10 μmol/L CB, 0.1 U/ml ADA, and increasing concentrations of CGS21680, NECA, or CCPA for an additional 5 minutes. Neutrophils were stimulated with 0.1 μmol/L fMLP in the presence of 1% ethanol for 10 minutes. The amount of radioactivity incorporated into [3H]PEt was assessed as described in “Materials and Methods” and is expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.519/6/bloo00203004x.jpeg?Expires=1735503707&Signature=a9zJDTFB1I5WSPnZyldjmFMj-M~PAWkHQRib2cORzX1yVGa6VimmauMGYSSSOf1~aHXmQLw4PaPIlxyHvlOGS41BRNnFftPkxJfTM4ssBINVHbtLS3GLkxESb9xYK7fJ6xw10D8xXygJrTfUFXIuRDFINC4x7YQTMqwtr0duv6n9C6LeCqdNY604uJY6GrQMdUhrpwwjBWdgzlXVJ32l6jmoZOAZoTpPeRUi8tHlGKGIJlnXshWoVK6HKoJr6BCs8Qwt69lRE~mVgbOs1vhpD1DyyXVPcPfKvKEjqEJa8wvZSC3BMDvj9R9iBj2ZZiKAX29yt71uvDin4-UUvmodYA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of Ado receptor agonists on fMLP-induced PLD activity.
1-O-[3H]alkyl-2-lysophosphatidylcholine-labeled neutrophils were preincubated at 37°C for 5 minutes. The cell suspensions were then pretreated with 10 μmol/L CB, 0.1 U/ml ADA, and increasing concentrations of CGS21680, NECA, or CCPA for an additional 5 minutes. Neutrophils were stimulated with 0.1 μmol/L fMLP in the presence of 1% ethanol for 10 minutes. The amount of radioactivity incorporated into [3H]PEt was assessed as described in “Materials and Methods” and is expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 3 separate experiments.
