![Fig. 6. The Ado receptor antagonists CGS15943 and CSC reverse the suppressive effect of CGS21680 on fMLP-stimulated PLD activity. / 1-O-[3H]alkyl-2-lysophosphatidylcholine-labeled neutrophils were prewarmed at 37°C for 5 minutes. (A) The cell suspensions were pretreated with 10 μmol/L CB and 0.1 U/mL ADA in the presence or absence of 0.1μmol/L CGS21680 for an additional 5 minutes. Where indicated, 0.1 and 1 μmol/L CGS15943 was also added to neutrophil suspensions 5 minutes before stimulation with 0.1 μmol/L fMLP for 10 minutes in the presence of 1% ethanol. (B) The experimental conditions were similar to A. Where indicated, 10 μmol/L CSC was added to neutrophil suspensions 5 minutes before stimulation with 0.1 μmol/L fMLP for 10 minutes in the presence of 1% ethanol. The amount of radioactivity incorporated into [3H]PEt was assessed as described in “Materials and Methods” and is expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.519/6/bloo00203006x.jpeg?Expires=1735504850&Signature=UER3AwjNtUwX8pB9KO~UL1Yo62iOKpCev1QVejF2KI77NJxJajuUoyNtOYbJc~nPO4WN1yAgixtbWmTRBNnX5~I6f9wcXuBW7BawGC0pxST6m91pAL7Qmttqn1kGA~6ssu8j0268N-kOK6VV-GVD-tgwlF2GDC4nBkOR5yhydMWHcl~ZN51mpGRqNf1j-e2bXeriMFCGGshfY~GBGQta3n4Ylvhz0TNuYvSB2rQyE41Pd~jrmJRDiP9qw3aptzuAKnjXdcG0M70Tjhnwf8EIGWgspzH~iQg0EE9h~uPVqW0ExoKIlYQEi4B9xWJFfm-cNjp~A4jgH06WnTuwGZepwQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The Ado receptor antagonists CGS15943 and CSC reverse the suppressive effect of CGS21680 on fMLP-stimulated PLD activity.
1-O-[3H]alkyl-2-lysophosphatidylcholine-labeled neutrophils were prewarmed at 37°C for 5 minutes. (A) The cell suspensions were pretreated with 10 μmol/L CB and 0.1 U/mL ADA in the presence or absence of 0.1μmol/L CGS21680 for an additional 5 minutes. Where indicated, 0.1 and 1 μmol/L CGS15943 was also added to neutrophil suspensions 5 minutes before stimulation with 0.1 μmol/L fMLP for 10 minutes in the presence of 1% ethanol. (B) The experimental conditions were similar to A. Where indicated, 10 μmol/L CSC was added to neutrophil suspensions 5 minutes before stimulation with 0.1 μmol/L fMLP for 10 minutes in the presence of 1% ethanol. The amount of radioactivity incorporated into [3H]PEt was assessed as described in “Materials and Methods” and is expressed as the percentage of the radioactivity in the total lipid extracts. The data are the means ± SEM of 3 separate experiments.
