The p85 subunit of PI3-kianse interacts with Tyro3 kinase domain.

(A) Purified GST-Tyro3 and GST alone used as a control were immobilized onto glutathione Sepharose beads and were incubated with NIH3T3 cell lysates. After washes, the proteins associated with GST-Tyro3 and GST beads were analyzed by SDS-PAGE and Western blotting using an anti-p85 antibody. Lane 1 is NIH3T3 total cell lysates. Lanes 2 and 3 are replicates of proteins bound to GST-Tyro3 beads. Lanes 4 and 5 are replicates of proteins bound to GST beads. (B) Purified GST-Tyro3 (lanes 1 and 2) and GST (lanes 3 and 4) were analyzed by SDS-PAGE. Lane 5 consists of protein standards.