Ectopic expression of EGFR-Tyro3 and Tyro3.

(A) NIH3T3 cells transfected with a full-length Tyro3 (lane 1), an EGFR/Tyro3 fusion receptor (lane 2), and a Tyro3/Trk-C fusion receptor (lane 3) were lysed, and equal amounts of cell lysates were analyzed for Tyro3 expression using an antibody recognizing the C-terminal part of the receptor. E/T denotes the EGFR/Tyro3 fusion receptor, and T denotes the Tyro3 receptor. T-st indicates a short form of Tyro3. (B) As a loading control, equal amounts of the cell lysates, as described in Figure 3A, were analyzed by SDS-PAGE and by Western blotting using an anti-P85 antibody. (C) Various cell lysates were immunoprecipitated with the anti-Tyro3 antibody, and immunoprecipitates were analyzed for Tyro3 expression.