EGFR/Tyro3 activation is associated with an increased PI3 kinase activity.

NIH3T3-EGFR/Tyro3 cells treated with EGF for various times were lysed. Equal amounts of cell lysates were immunoprecipitated with the anti-p85 antibody. Immunoprecipitates were blotted for (A) Tyro3 and (B) p85. (C) NIH3T3-EGFR/Tyro3 cells treated with EGF for various times were immunoprecipitated with the anti-Tyro3 antibody. Immunoprecipitates were analyzed for the PI3 kinase activity, as described in “Materials and methods.” The PIP3 portion of the thin-layer chromatography data was shown. Lane 1 is the PI3 kinase assay results (a positive control) using p85-immunoprecipitates from serum-stimulated cell lysates. EGF, epithelial growth factor.