PCR-SSCP analysis of BCL-6 mutations in representative B-cell neoplasms.

Representative results obtained for PCR-SSCP fragments E1.10 and E1.12 are shown. Samples of B-cell neoplasms are indicated at the top of each lane by a numbered code. A positive control (POS), represented by a tumor sample known to harbor BCL-6 mutations, as well as a normal (N) sample, represented by a lymphoblastoid cell line, are also included for each PCR-SSCP fragment shown. Samples were scored positive when their migration pattern differed from the normal control (N) and the migration abnormalities could not be ascribed to population polymorphisms. Among the samples shown in the figure, cases scored as positive included cases 1063 (primary splenic B-DLCL), 166 (primary extranodal B-DLCL), 1955 (primary central nervous system B-DLCL) for PCR product E1.10, and cases 998 (systemic de novo B-DLCL), 374 (systemic de novo B-DLCL), 1053 (primary splenic B-DLCL), 1054 (primary splenic B-DLCL), and 187 (primary extranodal B-DLCL) for PCR product E1.12.