Fig. 1.
The CD34/CD38 phenotype of Lin− CB cells changes during ex vivo transduction culture.
In this representative experiment (n = 3), uncultured (day 0) or cultured (days 1 to 4) cells were labeled with PerCP- and PE-conjugated mouse immunoglobulin G as isotype controls (upper panels) or anti-CD34-PerCP and anti-CD38-PE (lower panels) and analyzed by flow cytometry. Quadrants were set to locate at least 99% of the nonspecific events in the lower left.
