Fig. 2.
Gene transfer into the “expanded” CD34+CD38− subpopulation is comparable to that into the total cell population.
(A) After 4 days of ex vivo transduction culture, cells were labeled with anti-CD34-PerCP and anti-CD34-PE. R1 is a region defining the CD34+CD38−fraction. (B) A histogram shows EGFP fluorescence in the total cell population. (C) A histogram shows EGFP fluorescence within the CD34+CD38−fraction as defined by the R1 region shown in (A).