p27 protein and mRNA expression in CLL and MCL B cells.

Highly enriched neoplastic B cells from patients with CLL and MCL were used to prepare total protein lysates and total RNA (see Material and Methods). Total protein lysates were electrophoresed, blotted, and incubated with anti-p27 (A top row) or with anti-CDK2 (A, bottom row). cDNA transcribed from total RNA was amplified using oligonucleotides recognizing p27 (B, top row) or β2-microglobulin (B, bottom row).