Effect of the anti-vWf monoclonal antibody 6G1 on binding of the 39/34-kd vWf fragment to platelets.

(A) Specific binding of ¹²⁵I-labeled 39/34-kd vWf fragment (final concentration, 1 μg/mL) to washed platelets (final concentration, 5 × 10⁸/mL) in the presence of 6G1 in 30 minutes at 22°C. Nonspecific binding was determined in the absence of 6G1 (see Materials and Methods). (B) Specific binding of¹²⁵I-labeled 39/34-kd vWf fragment (final concentration, 1 μg/mL) to platelets in the presence of botrocetin and either the absence (squares) or presence (circles) of 6G1 (final concentration, 50 μg/mL).