Fig. 4.
Autoradiogram of immunoprecipitates obtained from stable COS-1 cells transduced with the viruses containing pLNCXRhAG using 2D10.
Logarithmically growing COS-1 cells were incubated with [3H]-mannose for 5 hours, and the cell lysates were subjected to immunoprecipitation with 2D10; the immunoprecipitates were then separated by SDS-PAGE under reducing conditions (for details, see Materials and Methods). Lane 1, stable COS-1 cells transduced with the viruses containing pLNCX vector alone; lane 2, stable COS-1 cells transduced with the viruses containing pLNCXRhAG. Arrow shows RhAG glycoprotein.