Fig. 5.
FACS profile of stable COS-1 cells transduced with the viruses containing pLNCXRhAG using 2D10.
COS-1 cells were incubated with 2D10 (10 μg/mL) containing 0.5% bovine serum albumin in PBS. After washing, the cells were stained with phycoerythrin-conjugated goat F(ab')2 anti-mouse IgG and then analyzed by flow cytometry (for details, see Materials and Methods). The solid line represents control cells (COS-1 cells transduced with the viruses containing vector alone), and the dotted line, COS-1 cells transduced with the viruses containing pLNCXRhAG.