Inhibition of JMML spontaneous CFU-GM colony growth by exposure of JMML mononuclear cells to L-739,749.

Exposure was in liquid suspension for either 1, 3, or 5 days before the establishment of colony assays. Cells were washed twice before the establishment of soft agar cultures to remove any extracellular L-739,749 in the liquid suspension. Results expressed are the percentage inhibition of maximal spontaneous CFU-GM colony growth, and they are the mean of experiments performed in triplicate from patient J49 with JMML. Concentrations of L-739,749 added are 1μmol/L (solid bars) and 10μmol/L (hatched bars).