T- and B-cell development in W/W chimeras.

Thymocytes and BM cells were depleted of red cells and stained with the indicated antibodies. Percentages are indicated within the dot plots. (A) Thymocyte numbers in W/W chimeras are much reduced, although thymopoiesis appears rather normal in the absence of c-kit. (B) BM pre-B (B220+ IgM−) and B cells (B220+ IgM+) are represented in normal percentages in W/Wchimeras. FACS profiles are representative of 4 independent experiments. (C) Reconstitution of splenic lymphoid populations with the presence of CD3⁺ T cells and B220⁺ B cells in W/W chimeras.