Phenotype of W/W NK cells.

Spleen cells were red-cell depleted and stained with the indicated FITC-labeled antibodies in combination with NK1.1^PE, TCRαβ^biot, and CD19^biot. An electronic gate was set to exclude TCRαβ⁺ T cells and CD19⁺B cells. The percentage of NK1.1⁺ NK cells positive for the indicated marker (white histograms) was calculated over the background of an isotype-matched control mAb (gray histograms). Results are representative of 3 independent experiments comprising 6 wt and 6 W/W chimeras.