Proliferation and cell survival of purified c-kit⁻ and c-kit+ NK cells.

Splenic NK cells were sorted by FACS in c-kit+ (○) and c-kit− (□) subsets, plated at 104 cells/well in CM supplemented with the indicated doses of IL-2 in the presence (•) or absence (○, □) of SCF (100 ng/mL). Plots indicate the numbers and percentages of live cells evaluated by trypan blue exclusion. Representative of 2 independent experiments.