Detection of the BCR-ABL^p190 fusion gene product in the bone marrow of NOD/SCID mice with human Ph¹-ALL grafts.

RT-PCR detection of the BCR-ABL^p190fusion gene using specific primers for both human BCRand ABL genes in engrafted NOD/SCID mice with 10⁵ cells (lane 1, patient No. 1; lane 2, patient No. 2; lane 3, patient No. 3; lane 5, patient No. 5; and lane 6, patient No. 6) and control NOD/SCID mice (lanes 4 and 7). The specificBCR-ABL^p190 fusion gene product was identified by hybridization with a specific oligo spanning the gene fusion. The cDNA integrity was measured by hybridization of the RT-PCR product using actin-specific primers with an internal actin human primer.