American Society of Hematology

Fig. 2.

$Fig. 2. Binding characteristics of the soluble GM-CSF receptor produced by human leukemic cell lines. / 125I-GM-CSF saturation binding experiments were performed on supernatant conditioned by the human leukemic cell lines shown. Before analysis the supernatants were subjected to ligand affinity column chromatography and eluted fractions were pooled, dialyzed against distilled H2O lyophilized and resuspended in 500 μL PBS. Shown are Scatchard analysis and saturation binding curves (inset) of representative experiments for U937 (A) (n = 6), THP-1 (B) (n = 5), and HL60 (C) (n = 2) cells.$

Binding characteristics of the soluble GM-CSF receptor produced by human leukemic cell lines.

¹²⁵I-GM-CSF saturation binding experiments were performed on supernatant conditioned by the human leukemic cell lines shown. Before analysis the supernatants were subjected to ligand affinity column chromatography and eluted fractions were pooled, dialyzed against distilled H₂O lyophilized and resuspended in 500 μL PBS. Shown are Scatchard analysis and saturation binding curves (inset) of representative experiments for U937 (A) (n = 6), THP-1 (B) (n = 5), and HL60 (C) (n = 2) cells.

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