Eosinophil rolling on VCAM-1 in vitro.
Eosinophils were infused at a flow rate of 0.7 and 1.4 dyn/cm2 into a parallel plate flow chamber containing VCAM-1– or BSA-coated coverslips. The number of rolling eosinophils (A) and adherent eosinophils (B) during continuous flow periods of 2 minutes were recorded and subjected to offline analysis. Results of experiments performed are presented as the mean ± SEM (n = 4 experiments). At flow rates of 0.7 dyn/cm2, significant numbers of eosinophils rolled on VCAM-1 (P = .001 vs BSA) and adhered to VCAM-1 (P = .001 vs BSA). At flow rates of 1.4 dyn/cm2, significant numbers of eosinophils rolled on VCAM-1 (P = .001 vs BSA), but the number of eosinophils adherent to VCAM-1 was significantly reduced. Solid bar, BSA; hatched bar, VCAM-1.
