Effect of anti-4, anti-β1, and anti-β7 integrin mAbs on eosinophil rolling on VCAM-1 in vitro.
Eosinophils (preincubated with either an anti-α4, anti-β1, anti-β7, anti-β1 and anti-β7 in combination, or control mAb) were infused at a flow rate of 0.7 dyn/cm2 into a parallel plate flow chamber containing VCAM-1– or BSA-coated coverslip. The number of rolling eosinophils during continuous flow periods of 2 minutes was recorded and subjected to offline analysis. Results of experiments performed are presented as the mean ± SEM (n = 3 experiments). (A) The anti-α4 mAb significantly inhibited eosinophil rolling on VCAM-1 (P = .01 vs control). (B) The anti-β1 and anti-β7 mAbs in combination (P = .01 vs control) as well as the individual anti-β1 mAb (P = .05 vs control) and anti-β7 mAb (P = .05 vs control) inhibited eosinophil rolling on VCAM-1.
