Fig. 6. 4β1 and 4β7 integrins support... | American Society of Hematology

Fig. 6.

$Fig. 6. 4β1 and 4β7 integrins support eosinophil rolling in postcapillary venules in vivo. / CFDA-labeled eosinophils were preincubated with either function-blocking anti-α4, anti-β1, anti-β7, or anti-β1 plus anti-β7 mAb in combination prior to the administration of eosinophils into the mesenteric microcirculation. The fraction of rolling eosinophils (Rf ) was determined in IL-1β–stimulated rabbit mesenteric venules (n = 5 to 12 rabbits). The ability of the different mAbs to block eosinophil rolling (% inhibition) was determined. Data represent mean ± SD. There was significant inhibition of eosinophil rolling induced by the anti-α4 mAb (P = .0001 vs control), the anti-β1 mAb (P = .001 vs control), the anti-β7 mAb (P = .004 vs control), and the combination of anti-β1 and anti-β7 mAbs (P = .0001 vs control) but not by the anti-β2 mAb (P = not significant vs control).$

4β1 and 4β7 integrins support eosinophil rolling in postcapillary venules in vivo.

CFDA-labeled eosinophils were preincubated with either function-blocking anti-α4, anti-β1, anti-β7, or anti-β1 plus anti-β7 mAb in combination prior to the administration of eosinophils into the mesenteric microcirculation. The fraction of rolling eosinophils (Rf ) was determined in IL-1β–stimulated rabbit mesenteric venules (n = 5 to 12 rabbits). The ability of the different mAbs to block eosinophil rolling (% inhibition) was determined. Data represent mean ± SD. There was significant inhibition of eosinophil rolling induced by the anti-α4 mAb (P = .0001 vs control), the anti-β1 mAb (P = .001 vs control), the anti-β7 mAb (P = .004 vs control), and the combination of anti-β1 and anti-β7 mAbs (P = .0001 vs control) but not by the anti-β2 mAb (P = not significant vs control).

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