Effect of anti-integrin mAb treatment on the velocity distribution profiles of rolling eosinophil in mesenteric venules.

The passage of rolling eosinophils in IL-1β–stimulated mesenteric venules was recorded. The velocity of consecutive rolling eosinophils was determined before and after eosinophil treatment with either anti-β1, anti-β7, anti-β1 plus anti-β7, anti-α4, or anti-β2 mAbs (n = 7 rabbits; 2 to 3 representative venules per rabbit). The velocity of rolling eosinophils (mm/sec) was manually determined by frame-by-frame analysis of recorded video images and represented as mean ± SD. The rolling velocity of eosinophils was increased by pretreatment of eosinophils with either anti-β1 (P = .004 vs control), anti-β7 (P = .005 vs control), anti-β1 plus anti-β7 (P = .003 vs control), and anti-α4 mAbs (P = .003 vs control) but not by pretreatment with anti-β2 mAbs.