Histogram plot of flow cytometric analysis.

TM antigen on the monocyte surface was detected by flow cytometry using unlabeled anti-TM mouse monoclonal antibody and a secondary, FITC conjugated antimouse antibody. The fluorescence intensity in the absence of the specific antibody (plot on the left) was set as a reference. Changes in fluorescence intensity on the horizontal axis reflect the specific TM labeling on the surface of the cells. The increase in fluorescence intensity associated with TM antigen expression was more pronounced in a normal sample (B) than in a sample from a carrier of the mutation (A).