Western blot of the expressed proteins.

SDP-PAGE and immunoblot of a truncated TM form (tTM) and of cell lysates from cells expressing wild type (wtTM), insertional mutant (ins T 1689) TM or no TM (mock). Immunodetection was performed with sheep polyclonal anti-TM antibody, followed by HRP-conjugated antigoat antibody and ECL detection. Panels represents proteins under nonreducing (left) and reducing (right) conditions as shown. The mol wt marker (middle) was always reduced, and following electroblotting was detected separately with streptavidin-HRP conjugate.