Adhesion to FN.

Adhesion to FN decreases portion of blood and BM CD34⁺ cells and CFC that are in S phase. CD34⁺cells from steady-state BM or mobilized PB were cultured for 48 hours in serum-free medium with low-dose cytokines. Cells were then plated in wells coated with PLL or FN for 12 to 16 hours. We collected adherent (Adh) and nonadherent (NA) cells separately. We analyzed the S phase of CFC by thymidine suicide assay and analyzed the S phase of all CD34⁺ cells by labeling cells with propidium iodide and analysis by FACS. (A) Thymidine suicide assay (n = 4 for BM and n = 4 for PB). Data are shown as mean ± SEM. Comparison between the FN-Adh and the FN-NA portion for PB or BM: * = P < .01. (B) FACS analysis of propidium iodide labeled cells (n = 12). A representative experiment is shown.