FISH analysis of deletions by using locus-specific probes.

Ph-positive metaphases from a patient with a variant Ph translocation t(9;12;22)(q34;q11;q11) were hybridized with probes from chromosome 9 (A and B) or 22 (C and D). (A) C106G1220 (red) and N14A7 (green) signals were observed on both the normal 22 and derivative 12 chromosome. (B) C106G1220 and N4A10 signals were seen on the normal 22, but only the C106G1220 signal was detectable on the derivative 12 chromosome. (C) D9S60 (red) and AK1 (green) signals were observed on both the normal 9 and the derivative 9 chromosome. (d) D9S60 and SPTAN1 signals were seen on the normal 9 chromosome, but only the D9S60 signal was detectable on the derivative 9 chromosome.