Cell-type–specific binding of sVCAM-1 to 4β1 integrin.

Jurkat, CHO-α4β1, RBL, THP-1, U937, or HUT-78 was resuspended in either DMEM containing 0.1% BSA (solid line), DMEM with the addition of 5 mmol/L EDTA (shaded line), or Tyrode's buffer containing 1 mmol/L MnCl₂ (dotted line). Seven Ig-domain VCAM-Ig (20 μg/mL) was added, and the mixture was incubated at room temperature for 30 minutes. VCAM-Ig binding was detected with FITC-conjugated donkey antihuman IgG as described in “Materials and Methods.” Results are representative of 3 separate experiments.