Cellular-energy depletion suppresses sVCAM-Ig binding.

Jurkat (A) or CHO-α4β1 (B) cells were placed in glucose-free Tyrode's buffer containing 2-deoxyglucose (2 mg/mL) and sodium azide (0.1% wt/vol) and were incubated at 37°C for 0, 30, 60, or 120 minutes. Subsequently, cells were resuspended in DMEM or Tyrode's buffer containing 1 mmol/L MnCl₂ (Mn⁺⁺), and 7 Ig-domain VCAM-Ig binding was assessed as described in “Materials and Methods”. VCAM-Ig binding was expressed as a percentage of initial binding in cells incubated in the absence of 2-deoxyglucose and sodium azide. Results are the mean ± SEM of 3 independent experiments. ◊, Mn⁺⁺ added; □, no addition.