RT-PCR and flow cytometric analyses of IL-15R, IL-2R, and control GAPDH.
(A) RT-PCR analysis of expression of IL-15Rα (upper) and control GAPDH (lower) in myeloma cell lines. The results indicated that all myeloma cell lines expressed the mRNA of IL-15Rα (778 bp) in 2 alternatively spliced isoforms. Lane 1: PBMC from a healthy donor as positive control; lane 2: erythrocytes from a healthy donor as negative control; lane 3: dH2O as technical negative control; lane 4: RPMI 8226; lane 5: OPM-2; lane 6: MC-Car; lane 7: LP-1; lane 8: IM-9; lane 9: ARH-77; and lane 10: the molecular weight marker pGEM (Promega). (B) Flow cytometric analysis of all constituents of the IL-15R and the IL-2R in RPMI 8226 cells. Cells were stained with antibodies specific for the relevant subunits of the receptors or the respective isotype-matched control mAb. Fluorescence intensities (FI) were determined by flow cytometry. Each histogram represents 3 independent measurements.
