Fig. 3.
Western blot analysis of plasma membranes of activated neutrophils.
Cells (20 × 106) were incubated with PMA (100 ng/mL) or without PMA for 10 minutes at 37°C. Fractionation of the cells was performed as previously described. PAGE (10%) was performed for the plasma membranes with comparable amounts of cytochrome b558. The blot was stained with rabbit antisera against p47-phox and p67-phox. The patients are indicated above the lanes. For each patient, material of a control donor was processed in parallel. The treatment (−PMA or +PMA) is also indicated above the lanes. From patient MK, we did not obtain enough neutrophils to investigate the unstimulated cells; therefore, only the +PMA lane is shown.
