Three cases of B-CLL with intermingled HRS-like cells.

(A, B, E) Anti-CD30 antibody staining of cases 1-3, hematoxylin counterstain. Single CD30-positive HRS-like cells are interspersed in the B-CLL infiltrated lymph node. (A) Case 1, 200 × magnification. (B) Case 2, 1000 × magnification. (E) Case 3, 400 × magnification. (C) Hematoxylin-eosin staining of case 2. The infiltrate mainly comprises small lymphocytes, a few blast cells, and histiocytes. A Reed-Sternberg–like cell is seen in the middle of the picture, 200 × magnification. (D) Hematoxylin-eosin staining of case 2. A Reed-Sternberg cell surrounded by small lymphocytes and histiocytes is seen in the middle of the picture, 1000 × magnification. (F) In situ hybridization of frozen section from CLL-infiltrated lymph node of case 3 for EBER-RNA. The HRS-like cells carry EBER-RNA, 400 × magnification. (A-F) Sections of formalin-fixed, paraffin-embedded, or frozen tissue.