Fig. 2.
Inhibition of secondary mixed lymphocyte reactions across minor histocompatibility barriers.
Responder spleen cells were harvested from primed B10.D2 mice 21 days after intraperitoneal immunization with 50 × 106irradiated (30 Gy) BALB/c spleen cells. 2.5 × 105primed spleen cells were plated with 5 × 105irradiated (30 Gy) BALB/c spleen cell stimulators in the presence or absence of PG27. After 96 hours of incubation, cultures were pulsed with 1 μCi [3H] thymidine/well for an additional 16 hours. Results are mean cpm from triplicate cultures and are representative of 2 separate experiments.