Engraftment of donor bone marrow in the B10.D2 → BALB/c transplant documented by polymerase chain reaction amplification of a polymorphic microsatellite region within the IL-1β gene.

Long-term engraftment of allogeneic bone marrow was found in all transplanted mice. This figure demonstrates a representative analysis of an ethidium bromide-stained 1.5% agarose gel. (lane 1) DNA marker φ × 174 digested with Hae III (marker sizes 1353, 1078, 872, 603, 310, 281 and 271, and 243, 194 bp; GIBCO BRL). (lane 2) Negative control (PCR without DNA). (lane 3) Recipient (BALB/c) standard. (lane 4) Donor (B10.D2) standard. (lanes 5–8) Recipients given PG27 (ID nos. 602, 604, 2011, 2012). (lanes 9–12) Recipients given solvent control (ID nos. 606, 610, 2006, 2007).