Adoptive transplantation.

The PG27 recipients treated 100 days after bone marrow transplantation were used as adoptive donors. Lethally irradiated (8 Gy) BALB/c mice were used as same-party adoptive recipients, and lethally irradiated (9.5 Gy) C3H mice were used as third-party adoptive recipients. 2.5 × 105 bone marrow cells and 10 × 106 spleen cells from adoptive donors were injected intravenously into BALB/c or C3H adoptive recipients. In control groups, normal B10.D2/nSnJ mice were used as donors, and the same numbers of bone marrow and spleen cells were transplanted in lethally irradiated BALB/c or C3H mice. These adoptive recipients were observed for 100 days without any treatment. (A) Survival curve. (B) Mean body weight curve. Data are pooled from 2 similar experiments.