Adhesion efficiency as a function of shear rate and ICAM-1 expression level.

The kinetics of heterotypic aggregation over the first 60 seconds of fMLP stimulation and initiation of shear were fit with a mathematical model at each shear rate, as described in “Materials and Methods.” Figure shows the efficiency of neutrophil capture of the parent 300.19 cell line and of transfected clones expressing low (I_low) and high (I_high) levels of ICAM-1. The experimental data were fit as smooth curves with a first-order exponential decay function. Data are presented as mean ± SEM from 3 to 7 separate experiments.