Contributions of CD11a and CD11b to neutrophil–ICAM-1 adhesion over the time course of fMLP stimulation.
Neutrophils (1 × 106 cells/mL) were preincubated with saturating concentrations of anti-CD11a, anti-CD11b, or both concurrently. They were then mixed with Ihigh(2 × 106 cells/mL), added to the cone-plate viscometer, and allowed to equilibrate for 2 minutes before stimulation with 1 μmol/L fMLP and initiation of fluid shear. Samples were taken at prescribed time points, fixed, and detected by flow cytometry as described in “Materials and Methods.” The contribution of each β2-integrin subunit to capture of 300.19–ICAM-1 was assessed under the following conditions: (A) Ilow at 90 s−1, (B) Ihigh at 90 s−1, and (C) Ihigh at 600 s−1. *P < .05 compared with control at the same time point. Data are presented as mean ± SEM from 3 to 6 separate experiments.
