Fig. 8.
Fig. 8. Strength of adhesion through CD11a/CD18 and CD11b/CD18. / Neutrophils (1 × 106 cells/mL) were preincubated with anti-CD11a (▾) or anti-CD11b (⋄) for 10 minutes at saturating concentrations and then mixed with 300.19 Ihigh cells (2 × 106 cells/mL). Suspensions were stimulated with 1 μmol/L fMLP and sheared in the cone-plate viscometer over a range of shears. Peak extent of heterotypic aggregation in the presence of anti-CD11b or anti-CD11a was measured and plotted at each shear stress. Aggregation is compared for experiments performed in (A) normal HEPES buffer of viscosity 0.7 cp or (B) buffer of viscosity 1.7 cp augmented with 6% Ficoll. Data are presented as mean ± SEM from 3 to 6 separate experiments.

Strength of adhesion through CD11a/CD18 and CD11b/CD18.

Neutrophils (1 × 106 cells/mL) were preincubated with anti-CD11a (▾) or anti-CD11b (⋄) for 10 minutes at saturating concentrations and then mixed with 300.19 Ihigh cells (2 × 106 cells/mL). Suspensions were stimulated with 1 μmol/L fMLP and sheared in the cone-plate viscometer over a range of shears. Peak extent of heterotypic aggregation in the presence of anti-CD11b or anti-CD11a was measured and plotted at each shear stress. Aggregation is compared for experiments performed in (A) normal HEPES buffer of viscosity 0.7 cp or (B) buffer of viscosity 1.7 cp augmented with 6% Ficoll. Data are presented as mean ± SEM from 3 to 6 separate experiments.

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