PCR detection of the MiniAdFVIII vector in vivo.

(A) Primers used to detect junctions between critical fragments in the MiniAdFVIII genome. Five pairs of primers (F = forward; R = reverse) and their sequences are listed. The positions of the primers are marked as the nucleotide length (kb) along the MiniAdFVIII genome. (B) Copies of the vector DNA per cell equivalent were determined at 5 positions along the length of the MiniAdFVIII genome. Locations of the primers are mapped at the top of the panel, and the position of detected segments along the vector genome are indicated in kb on the X-axis.