Fig. 3.
Effect of H7 on IL-4-induced Stat6 tyrosine phosphorylation and DNA binding.
(A) HepG2 (left) and Ramos cells (right) were untreated or treated with H7 (100 μmol/L) for 30 minutes and then stimulated with IL-4 (100 ng/mL) for 10 minutes as indicated. Stat6 was immunoprecipitated and separated by 7.5% SDS-PAGE and was subjected to anti-phosphotyrosine immunoblotting. Shown in the lower panel are protein levels after anti-Stat6 reblotting. (B) Lysates from Ramos cells (left) and COS-7 cells transfected with Stat6 (right), treatments as in (A), and nuclear lysates were analyzed in the mobility shift assay using32P-labeled Igε oligonucleotide. The Stat6 binding complex is indicated with an arrow.
