IL-4-induced activation of Igɛ-RE reporter is inhibited by H7 but not by wortmannin.

(A) HepG2 cells were transfected with Igε-RE reporter construct (0.5 μg) together with a β-galactosidase vector (0.2 μg). Forty-eight hours after transfection, the cells were treated with IL-4 (10 ng/mL), H7 (200 μmol/L), or wortmannin (1 μmol/L) as indicated for 6 hours, and β-galactosidase and luciferase activities were measured. The luciferase values were normalized against β-galactosidase. Mean values of 3 independent experiments with SD are shown. (B) HepG2 cells transfected with Igε-RE reporter and treated with IL-4 and different concentrations of H7 for 6 hours, as indicated, and luciferase and β-galactosidase activities were measured. (C) Human 293T cells were transfected with Stat6 expression vector (0.15 μg), together with Igε-RE and β-galactosidase vectors. H7 and IL-4 treatments were performed as described above, and luciferase and β-galactosidase values were measured.