American Society of Hematology

Fig. 5.

$Fig. 5. Compartmentalization of phosphorylated Lyn kinase with PI3K in lipid bodies of human PMN leukocytes. / PAF-pretreated PMN leukocytes were stimulated with GM-CSF (60 ng/mL) for 10 minutes before subcellular fractionation. Proteins from the lipid body and other subcellular fractions (20 μg each) and a PMN leukocyte cell lysate (200 μg) were electrophoresed and transferred to membranes for immunoblotting. (A) Phosphotyrosine proteins were detected with antiphosphotyrosine mAb 4G10. PI3K p85 (B) was detected with anti-PI3K p85 mAb, and Lyn kinase (C) was detected with an anti-Lyn kinase pAb.$

Compartmentalization of phosphorylated Lyn kinase with PI3K in lipid bodies of human PMN leukocytes.

PAF-pretreated PMN leukocytes were stimulated with GM-CSF (60 ng/mL) for 10 minutes before subcellular fractionation. Proteins from the lipid body and other subcellular fractions (20 μg each) and a PMN leukocyte cell lysate (200 μg) were electrophoresed and transferred to membranes for immunoblotting. (A) Phosphotyrosine proteins were detected with antiphosphotyrosine mAb 4G10. PI3K p85 (B) was detected with anti-PI3K p85 mAb, and Lyn kinase (C) was detected with an anti-Lyn kinase pAb.

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